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Image Search Results
Journal: PLoS ONE
Article Title: Norspermidine and Novel Pd(II) and Pt(II) Polynuclear Complexes of Norspermidine as Potential Antineoplastic Agents Against Breast Cancer
doi: 10.1371/journal.pone.0055651
Figure Lengend Snippet: Twenty-four h after seeding of L56Br-C1 cells, NSpd, Pd-NSpd or Pt-NSpd was added to give a final concentration of 25 µM. After 72 h of treatment, cells were harvested for SCGE analysis. The ethidium bromide-stained nucleoids were photographed and then examined using the Comet Score™ Freeware. A . Images of comets obtained by the SCGE assay. DNA damage results in comets with head and tail, whereas undamaged DNA results in a round head. B . Percentage DNA in tail on the x-axis versus tail length on the y-axis for individual cells. C . Tail moment TMOM (%DNA in tail multiplied by tail length) for individual cells. Data were collected from three independent experiments, n = 207 cells. D . Table showing the mean TMOM value of the 10% highest TMOM values i.e. 20 highest values ± SD. *p<0.05 compared to control; ***p<0.001 compared to control.
Article Snippet: For each sample, a total of 207 cells were analyzed for tail length and % DNA in tail using
Techniques: Concentration Assay, Staining, Control